Biologia molecolare - il sistema Entrez

Il sistema Entrez

• OMIM
• PubMed
• Giornali elettronici
• Mappe e genomi
• Sequenze nucleotidiche
• Strutture 3D
• Sequenze proteiche
• Tassonomia

NCBI

• Site Map
• Guide to NCBI resources
• Entrez Help
• help documentation for the Entrez system
• Entrez Global Query
• search a subset of Entrez databases
• Batch Entrez
• upload a file of GI or accession numbers to retrieve sequences
• E-Utilities
• Entrez programming utilities
• Literature Databases
• PubMed, OMIM, Books, PubMed Central
• NCBI Handbook
• In-depth guide to NCBI resources
• LinkOut
• external links to related resources
• Literature Databases
• PubMed, OMIM, Books,

Entrez is the text-based search and retrieval system used at NCBI for the major databases, including PubMed, Nucleotide and Protein Sequences, Protein Structures, Complete Genomes, Taxonomy, and others.

The complete list of Entrez databases can be viewed in the search pulldown menu.

Nucleotide Databases

• dbEST
• dbGSS
• dbSNP
• MGC
• PopSet
• RefSeq

Bookshelf

Look for background information or research new topics with freely accessible, online biomedical textbooks. The growing NCBI Bookshelf can be searched directly or accessed via PubMed abstracts by clicking the "Books" link. The database can also be searched by choosing "Books" from the Entrez pull-down menu.

PubMed

Through PubMed, anyone can access MEDLINE's 11,000,000 biomedical journal citations to research biomedical questions. Clicking the "Related articles" link for each abstract can expand your search. Click here for the PubMed tutorial.

PubMed Central

PubMed Central is a digital archive of life sciences journal literature. Integrated into the Entrez retrieval system, PMC provides free and unrestricted access to the full text of over 100 life sciences journals, with more to come.

Online Mendelian Inheritance in Man (OMIM)

With over 14,000 entries, OMIM, maintained by Dr. Victor A. McKusick and his colleagues at Johns Hopkins University, represents a comprehensive and constantly updated catalog of inherited diseases.

Correct base-pairing at the 3 end of PCR primers is the basis of allele-specific PCR.

Human Molecular Genetics

Tom Strachan & Andrew P. Read

6. PCR, DNA sequencing and in vitro mutagenesis

1. Basic features of PCR
2. Applications of PCR
3. DNA sequencing
4. In vitro site-specific mutagenesis

Design allele-specific primers (nucleotides 1-17)

3' Allele 1-specific (ASP1) primer

3' Allele 2-specific primer (ASP2)

PCR with ASP1 or ASP2 + conserved primer (CON)

Allele 1 DNA

CON

but

No amplification

Allele 2 DNA